Journal of Agriculture
and Environmental Sciences

A Modified and Efficient Method for Economical and Rapid Extraction of Genomic Dna for Marker-Assisted Selection in Wheat Hybridization
Muhammad Ejaz, Zhang Gaisheng, Zhao Huiyan

Abstract
Marker-assisted selection (MAS) for cloning in crop breeding and genetic studies is an important tool to improve crop plants. For that, isolation of good quality DNA from a huge population of plants require in a short period of time. Metabolites interfere with DNA isolation procedure and yield poor quality DNA. Here we present a modified and efficient DNA extraction technique that is reliable free from protein, polysaccharide and polyphenols contaminants from wheat crop. The method involves using CTAB and high salt concentration to precipitate the polysaccharides. A₂₆₀/A₂₈₀ and A₂₆₀/A₂₃₀ ratios were calculated from the spectrophotometric readings to confirm the purity of genomic DNA. The approach is fast, re-producible, and can be practical for PCR base markers identification, such as AFLP marker.

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