Journal of Agriculture
and Environmental Sciences

Bioactive compounds in aqueous infusions of dietary supplements and herbal blends containing dried hawthorn fruits or hawthorn inflorescences (Crataegus spp.)
Anna Przybylska, Grzegorz Bazylak

Abstract
The aim of this study was the preliminary analysis of micronutrient and phytochemical composition as well as antioxidant activity of aqueous extracts obtained from dietary supplements and herbal blends containing dry hawthorn fruits (HF) and hawthorn inflorescences (HI). The study included 15 samples. Total phenols (TPC) and flavonoids content (TFC) of aqueous extracts were determined using colorimetric assay. The antioxidant activity was analyzed by using DPPH and ABTS methods. To analyze the concentration of citric and L-malic acid were used enzymatic analysis, while to analyze of tartaric acid was used colorimetric method. Ascorbic acid and oxalic acid were determined using titration method. Mean TPC and TFC for aqueous extracts with dry HI is nearly 2.5 and 4.0 times higher than in extracts with dry HF, respectively. Results shows that TPC, TFC is positively and highly correlated with fragmentation degree of dry HF. High correlation was observed between TFC and DPPH and ABTS values, while no correlation observed between TPC and antioxidative activity with DPPH and ABTS assays of HF. The analysis of principal component analysis demonstrates different clusters based on the chemical composition separating aqueous extracts with HF from the HI.

Full Text: PDF     DOI: 10.15640/jaes.v7n2a14